Association of DCC, MLH1, GSTT1, GSTM1, and TP53 gene polymorphisms with colorectal cancer in Kazakhstan
Дата
2014-09-15Автор
Djansugurova, Leyla
Khussainova, Elmira
Kaidarova, Dilyara
Zhunussova, Gulnur
Iksan, Olzhas
Afonin, Georgiy
Parker, M. Iqbal
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Показать полную информациюАннотации
This study presents the first results of a moleculargenetic
study of colorectal cancer (CRC) in Kazakhstan.
Blood samples were collected from patients diagnosed with
rectal or colon cancer (249 individuals) as well as a control
cohort of healthy volunteers (245 individuals), taking into
account the age, gender, ethnicity, and smoking habits of the
CRC patients. Combined analysis of data obtained from individuals
of either Kazakh or Russian decent showed a significant
association with increased CRC risk in the following
genotypes: DCC (32008376G/G and G/A versus A/A; OR=
3.45, 95 % confidence interval (95 %CI)=1.75–6.81, χ2
=
14.07, p<0.0002), MLH1 (-93G/G versus G/A and A/A;
OR=1.45, 95 %CI=1.02–2.07, χ2
=4.21, p<0.04), TP53
(Pro72Pro; OR=3.80, 95 %CI=2.46–5.88, χ2
=61.27,
p<0.0001), combination GSTT1 deletions with heterozygotes
versus normal homozygotes (OR=1.43, 95 %CI=1.00–2.04,
χ2
=3.90, p<0.05), and GSTM1 deletions (OR=1.83,
95 %CI=1.28–2.63, χ2
=11.04, p<.001). Analysis for ethnicity
and smoking for each of the investigated polymorphisms
showed that some genotypes can have a predictive value for
susceptibility to CRC, at least those that demonstrate
statistically significant ORs either for the combined mixed
population of Kazakhstan or for both main ethnic groups
separately (Kazakhs and Russians): TP53 Pro72Pro homozygous
(for Kazakh—OR=3.40, 95 %CI=1.63–7.06, χ2
=
11.35, p<0.003; for Russian—OR=4.69, 95 %CI=2.53–
8.66, χ2
=53.19, p<0.0001) and GSTM1 deletions (for Kazakh—OR=2.30,
95 %CI=1.21–4.40, χ2
=8.42, p<0.01; for
Russian—OR=1.64, 95 %CI=1.01–2.66, χ2
=7.82, p<0.02).